Fig. 2

ZCWPW1 physically interacts with multiple HDAC proteins. a Schematic diagram illustrating the experimental design for the RIME analysis. b Venn diagram showing the overlap of proteins binding to ZCWPW1 among the three repeats (Rep1, Rep2, and Rep3) in the RIME analysis. c Example of putative ZCWPW1-interacting proteins. The proteins are associated with chromatin regulation and DNA repair. d Co-IP analysis of ZCWPW1 binding proteins from PD14–PD16 testes protein extracts in WT and Zcwpw1^–/– mice. HDAC1, HDAC2, and HDAC6 were immunoprecipitated with ZCWPW1. Data are representative of three independent experiments. e Co-IP analysis of ZCWPW1 binding proteins from PD14–PD16 testes protein extracts in WT mice. SMCHD1 was immunoprecipitated with ZCWPW1. Data are representative of three independent experiments. f Co-IP analysis of the interaction between ZCWPW1 and HDAC1, HDAC2, and HDAC6 in HeLa cells overexpressing ZCWPW1 for 48 h. Data are representative of three independent experiments