Fig. 4

Dyskerin binds to a complex RNA interactome in the cytoplasm. A Left: Biotype distribution of transcripts identified by RIP-Seq analysis of RNAs co-immunoprecipitated by an anti-dyskerin antibody in a cytoplasmic MCF7 lysate. Middle: Biotype distribution of H/ACA mRNAs immunoprecipitated by dyskerin from MFC7 cytoplasmic fractions. The plot shows also transcripts with or without SNORA sequence Right: Quantitative distribution of transcripts immunoprecipitated by dyskerin from MFC7 cytoplasmic fractions. The dotted horizontal line represents the median. Five independent replicates were used for RIP-seq analysis. B Fold enrichment (left) and FDR p-values (right) of the molecular function gene ontology chart of the statistically significant terms obtained from the analysis of the 238 unique protein coding transcripts immunoprecipitated by dyskerin. FDR, False Discovery Rate. C Representative Western blot analysis image (left) and densitometric analysis of 5 independent replicates (right) of dyskerin levels after DKC1 mRNA RNAi in MCF7 cells. Data are shown as means + SD. Paired Student’s t tests were performed relative to controls. Right: RT-qPCR analysis of transcripts identified by RIP-Seq analysis belonging to the most enriched molecular functions obtained by gene ontology analysis. DKC1 mRNA silencing level is highlighted in red. Data are shown as a fold change of DKC1 RNAi MCF7 cells relative to their controls. The means from three biological replicates (n = 3) are displayed, error bars represent SD. Paired Student’s t tests were performed relative to not DKC1 interfered controls. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.001.