Development of a highly efficient prime editor 2 system in plants

Table 1 Prime editing by optimized pPE2 systems in transgenic plants

Targets	pPE variants	pegRNA structures	pegRNA promoters^a	Tested lines^b	Precisely edited plants^c			Plants with byproduct^d
Targets	pPE variants	pegRNA structures	pegRNA promoters^a	Tested lines^b	Ho	He	Total (%)	Plants with byproduct^d
ACC-T	pPE2	pegRNA	OsU3	48	0	0	0 (0.00%)	0
	pPE2	pegRNA-evoPreQ₁	OsU3	48	0	1	1 (2.08%)	0
	pPEmax	pegRNA-evoPreQ₁	OsU3	48	0	7	7 (14.58%)	0
	pPEmax	pegRNA-evoPreQ₁	U6 composite	48	0	34	34 (70.83%)	1
PDS-T	pPE2	pegRNA	OsU3	48	0	4	4 (8.33%)	0
	pPE2	pegRNA-mpknot	OsU3	48	0	5	5 (10.42%)	0
	pPE2	pegRNA-evoPreQ₁	OsU3	48	0	10	10 (20.83%)	0
	pPEmax	pegRNA-evoPreQ₁	OsU3	48	0	14	14 (29.17%)	0
	pPEmax	pegRNA-evoPreQ₁	U6 composite	48	10	21	31 (64.58%)	4
ALS-T	pPE2	pegRNA	OsU3	48	0	1	1 (2.08%)	0
	pPE2	pegRNA-mpknot	OsU3	48	0	8	8 (16.67%)	0
	pPE2	pegRNA-evoPreQ₁	OsU3	48	0	23	23 (47.92%)	0
	pPEmax	pegRNA-evoPreQ₁	OsU3	48	8	24	32 (66.67%)	0
	pPEmax	pegRNA-evoPreQ₁	U6 composite	48	16	20	36 (75.00%)	2
CDC48-T	pPE2	pegRNA	OsU3	48	0	14	14 (29.17%)	0
	pPE2	pegRNA-mpknot	OsU3	48	0	12	12 (25.00%)	0
	pPE2	pegRNA-evoPreQ₁	OsU3	48	0	24	24 (50.00%)	0
	pPEmax	pegRNA-evoPreQ₁	OsU3	48	8	22	30 (62.50%)	0
	pPEmax	pegRNA-evoPreQ₁	U6 composite	48	19	18	37 (77.08%)	0

He, heterozygous mutants; Ho, homozygous mutants
^aU3, OsU3 promoter; U6 composite, CaMV 35S enhancer-CmYLCV-U6 composite promoter
^bFor each vector, 48 independent lines were randomly selected for genotyping
^cThe number of transgenic plants harboring precise edits
^dThe number of plants carrying undesired mutations in the targeted region

ISSN: 1474-760X