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Fig. 8 | Genome Biology

Fig. 8

From: PtoNF-YC9-SRMT-PtoRD26 module regulates the high saline tolerance of a triploid poplar

Fig. 8

SRMT and PtoNF-YC9 co-regulates the expression of PtoRD26 in response to salt stress. A The salt tolerance of SMRT-RNAi/PtoNF-YC9-RNAi, the double transgenic poplars (L18 and L31) in which the SRMT was knocked down in the background of PtoNF-YC9-RNAi-L17 compared to the SRMT-RNAi, PtoNF-YC9-RNAi, and WT poplars. B The MDA content of different lines after salt treatment. C The EL of different lines after salt treatment. The means ± s.d. of all data from at least three biological replicates are shown. Asterisks indicate statistically significant differences (two-sided Student’s t test, *P < 0.05, ns = not significant). D Western blotting indicates that the GFP abundance, driven by PtoRD26 promoter, was enhanced by expressing PtoNF-YC9.1-MYC, SRMT.3-FLAG, or co-expressing these two fused proteins. E The dual-luciferase assay indicates that LUC, driven by the artificial promoter constructed by fourfold ME4 repeats fused with the 35S minimal promoter, was significantly enhanced by co-expressing SRMT.3 and PtoNF-YC9.1 rather than expressing these two genes separately. F The working model of SRMT, PtoNF-YC9 and PtoRD26 in the ABA-dependent salt-stress response. The question marks indicate the unknown proteins. The gray arrows indicate the low expression levels of SRMT and PtoRD26, and the back bold arrows indicate the significantly enhanced expression levels of SRMT and PtoRD26

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