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Fig. 6. | Genome Biology

Fig. 6.

From: PtoNF-YC9-SRMT-PtoRD26 module regulates the high saline tolerance of a triploid poplar

Fig. 6.

SRMT interacts with PtoNF-YC9 in vitro and in vivo, and ABA signaling mediates shuttling PtoNF-YC9 from cytoplasm to nucleus. A The physical interaction between SRMT.3 and PtoNF-YC9.1 was investigated by Y2H assay. B The pull-down assay showed GST-PtoNF-YC9.1 to interact with the 6 × HIS tagged SRMT.3 (6 × HIS-SRMT.3) in vitro, and the pulled-down content of SRMT.3 increased with the abundance of SRMT.3 during incubation, whereas the GST protein as the control could not pull-down the SRMT.3 at all. C The Co-IP assay displayed that the FLAG tagged SRMT.3 (SRMT.3-FLAG) could interact with GFP-tagged PtoNF-YC9.1 (PtoNF-YC9.1-GFP) in vivo. D The transient transformation of poplar mesophyll protoplasts with GFP-tagged PtoNF-YC9.1 indicates that the GFP-PtoNF-YC9.1 localized both in the cytoplasm and nucleus without ABA treatment, while this recombination protein was more abundant in the nucleus than in cytoplasm after ABA treatment. However, the subcellular localization of free GFP was not changed after ABA treatment. E Western blotting indicates that the FLAG tagged PtoNF-YC9.1 (PtoNF-YC9.1-FLAG) to be more abundant in the nucleus after ABA treatment than without ABA treatment. F The abundance of PtoNF-YC9.1-FLAG in nucleus and cytoplasm with or without salt stress

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