Fig. 1
From: An ORFeome of rice E3 ubiquitin ligases for global analysis of the ubiquitination interactome
Construction of a ubiquitin E3 ligase-encoding ORFeome library. a Number of different types of E3 ligase genes in the rice genome. b Functionally characterized E3 ligase genes (69) in rice. c Number of synthesized and RT-PCR amplified E3 ligase genes. The Y-axis represents the number of synthesized and RT-PCR amplified genes. d Flowchart and timeline for Y2H screening of E3 ligases. The bait is inserted into the pGBKT7 (BD) vector and transformed into yeast strain AH109. The E3 ubiquitin ligase library is transformed into yeast strain Y187. e Identification of multiple E3 ligases from the rice E3 ligase library using OsUBC14 as the bait