Fig. 5

GoPeaks has a favorable specificity and sensitivity for broad H3K4me1 CUT&Tag peaks. a ROC curves quantifying the recall and false positive rates and b PR curves quantifying the precision and recall rates of H3K4me1 CUT&Tag data from H3K4me1 ChIP-seq data. Both ChIP-seq and CUT&Tag datasets were generated in K562 cells. Colors indicate the peak calling method. c Overlap of high-confidence peaks identified by each peak caller. d Annotation of unique peaks identified by each peak caller. Each bar is labeled by the number of unique peaks. Colors indicate the genomic feature. Downstream is at least 300 bp towards 3′ end of DNA strand. e, f Example peaks at the e. FOXO3 gene and f. FTCD genes. IgG replicates are the negative controls. Consensus peak calls for each method are shown. Tracks are CPM normalized and are scaled to the range [0–1.31] for e. and [0–1.33] for f. by IGV. Tracks are depicted on the GRCh38 genome assembly. UTR, untranslated region