Fig 6

6mA profiling at single-base resolution in the bacterial and eukaryotic genomes. a Workflow of DNA 6mA immunoprecipitation followed by NT-seq (6mA DIP-NT-seq). 6mA modified DNA fragment is labeled in green. b Bar plot of percentage of 6mA sites passing filter by different sequencing depth threshold. 100% at threshold 0 means there is no filtering. c Violin plot shows 6mA DIP increases the A to G ratio difference between 6mA and A. d Receiver operating characteristic (ROC) curve shows that 6mA DIP significantly improves the performance of 6mA detection at single-base resolution. e Pie charts of DIP-NT-seq in 6mA sites correctly and incorrectly classified by SMRT-seq. Normalized A to G ratio threshold was determined by maximizing the F1 score. f 6mA DIP-seq peak number in WT and MTA1 mutant Oxytricha strain. g SMRT-seq 6mA percentage in WT and MTA1 mutant Oxytricha strain. h DIP-NT-seq 6mA percentage in WT and MTA1 mutant Oxytricha strain. i IPD ratio of DIP-NT-seq undetected and detected 6mA. Statistic tests were performed by two-sided Mann-Whitney-Wilcoxon (MWW) test with Bonferroni correction (****: P ≤ 1.0e−6)