Fig. 3

Nanopore sequencing of human 18S rRNA from HEK293 cells. A UMAP projection of JACUSA2 base substitution, insertion, and deletion scores for uridine positions. psU stands for Ψ modified positions, Um stands for 2 ′-O-methyl ribose modifications and m1acp3psU stands for 1-methyl-3-(3-amino-3-carboxypropyl)pseudouridine-5 ′-monophosphate. B Line plot showing by ascending sum of JACUSA2 call-2 scores the number of predictions in green and the overlap with annotated uridine modifications in brown. Modified positions as defined by Taoka et al. [19]