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Fig. 1 | Genome Biology

Fig. 1

From: RNA modification mapping with JACUSA2

Fig. 1

Summary of JACUSA2 results on MazF restriction enzyme assay and DARTseq assay in HEK293 cells. A Synthetic RNA Oligo digestion and sequencing. Position 8 hosts the central A/m6A. Position 1-7 and 9-16 are random. We have sequenced 2 libraries, which differ in position 8 (A/m6A). The MazF recognition motif becomes apparent when we consider cleaved fragments (3 ′ 8bp fragments). B Arrest rate analysis across replicates in published data from HEK-293 cells [9]. Results are shown for a set of 2792 well covered sites with robust and significant read arrest changes. One-sided Wilcoxon signed-rank test: Maz-FTO-ACA vs. MAZ-mock-ACA; one-sided Wilcoxon rank sum test pvalue <10−16. C Comparison of JACUSA2 rt-arrest predictions for [9] to a high-confident set of miCLIP sites (intersection of 3 miCLIP experiments [1517]) on gene set level. D Comparison of JACUSA2 call-2 predictions for [11] to a high-confident set of miCLIP sites (intersection of 3 miCLIP experiments [1517]) on gene set level. E cDNA distance of closest miCLIP site (intersection of 3 miCLIP experiments) to the respective MazF cleavage site (red) or DART C →U site (turquoise). Wilcoxon rank sum test pvalue: 5.957e−13

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