Fig. 2

Mismatch error rate for nanopore long reads with different repeat lengths. Repeat regions are highlighted in yellow. a and b have 76 and 8 G4C2 repeat copies for 2 synthetic sequences respectively, while c and d have ~ 800 and 15 repeat copies for a BAC clone. Each dot is for an averaged mismatch error of a 50 bp region to reduce randomness. The long-read data were released by [37] and rebasecalled via Guppy v3.3.3 (solid lines) and Guppy 5.0.7 (dashed lines). A summary of error rate statistics is shown in Additional file 3