Fig. 4

MC2-sensitive detection of rare behavior. A Correlation matrix between log gene expression frequency of 260 genes with rare expression signatures (see text). We highlight several gene clusters at the left. B Each bar graph show specificity (left) and fold change enrichment (right) of top genes separating three exemplified rare transcriptional behaviors. Also shown for each rare behavior are the distribution of total gene expression of rare genes per single cell within the top-enriched metacell(s) (shades of green) and within the top enriched PCA+Louvain subcluster (orange). C Shown are single-cell gene expression for rare behavior marker genes and for genes correlated and anticorrelated with them, plotted for cells within the most strongly enriched PCA+Louvain sub-cluster for the observed behavior