Fig. 7

Mismatch resolution and flap asymmetry.a Sketch of the construct and of the guide RNAs used to induce double-strand breaks at alternative locations. b Global bias reversal. The dot plot shows the bias toward T with the G:T construct, measured by UMI-PCR 24 h after inducing a double-strand break with the guide RNAs shown in a. c Local bias reversal. The circular map of the mouse genomes shows a random sample of 1000 inserted G:T reporters where the final state after repair was known with both guide RNAs shown in a. Each tick mark represents an integrated reporter, and its color represents the outcome of both experiments, as per the heat map in the top right corner. Most tick marks are magenta, indicating that the reporter was repaired toward G when using guide RNA #1 and toward T when using guide RNA #2