Fig. 4

Global differences between reporters. a Repair across replicates. Each row shows a random barcode from the G:T construct, and each column shows a replicate where it appears. The color of the rectangle indicates the repair outcome. Without I-SceI induction (Mock), barcodes are typically present in more than two replicates, always with the same outcome. Twenty-four or 48 h post-I-SceI induction, barcodes are typically present in two replicates with different outcomes. The results are similar for all the constructs (not shown). b Amplification of unrepaired mismatches. If the mismatch is unrepaired, UMI-LA (left) produces UMIs with the allele of the top strand only, whereas UMI-PCR produces UMIs with both alleles. c Conflicts among UMIs. The dot plot shows the proportion of barcodes such that at least one UMI reports a different allele than the majority. Colors and symbols are the same as in Fig. 3b. d Mutual information between replicates. The bar graph shows the average mutual information per barcode between pairs of replicates (experiments with the T:C construct had only 100 pairs, compared to > 1000 pairs for the other constructs and the no I-SceI controls)