Fig. 3

Measure of repair biases. a Quantification methods. In UMI-LA (left), barcoded reporters are amplified by 50 cycles of linear amplification using a primer decorated by UMIs. In UMI-PCR (right), reporters are amplified by 6 PCR cycles where one primer is decorated by UMIs. Either way, the products are further amplified by regular PCR. After sequencing, each barcode is associated with several UMIs, themselves associated with alleles. Repair biases are quantified by giving one vote per UMI. b Repair outcome. The dot plot shows the measured bias toward A or T (whichever applies) in each technical replicate. For each construct, data points obtained 24 and 48 h post I-SceI induction are shown on the left and on the right, respectively. c Graphical summary of the results. The nucleotide of the top strand is most frequently kept during the resolution of the mismatch