Fig. 6

Prime-seq is very cost-efficient. A With a set budget of $500, prime-seq allows one to process 198 samples, which is 1.6 times more samples than the next cost-efficient method. B The compared methods were grouped into low, middle, and high cost methods and the TruSeq MAQCII data was used as a basis for power analysis for all methods but prime-seq. The increase in sample size due to cost efficiency directly impacts the power to detect differentially expressed genes, as evident by the increased performance of prime-seq and other low cost methods (BRB-seq and Decode-seq), even when sequencing costs are included in the comparison (sequencing depth of 10 mio. reads at a cost of $3.40 per 1 mio. reads)