Fig. 4

RNA extraction with beads, rather than columns, provides similar sequencing data while increasing throughput capabilities. A Feature distributions of RNA isolated with a column-based kit and magnetic beads show that both RNA extraction protocols produce similar amounts of useable reads from cultured human embryonic kidney 293T (HEK293T) cells, peripheral blood mononuclear cells (PBMC), and harvested mouse brain tissue. B Gene expression between both bead and column extraction are also similar in all three tested inputs (R² = 0.86 HEK, 0.84 PBMCs, and 0.74 tissue). C Detected UMIs and detected genes for column and magnetic beads in HEK293T, PBMCs, and tissue are almost identical, with slightly more detected genes in the bead condition (filtering parameters: detected UMI ≥ 1, detected gene present in at least 25% of samples and is protein coding). Comparison of costs (D) and time (E) required for different RNA extractions