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Fig. 1 | Genome Biology

Fig. 1

From: Prime-seq, efficient and powerful bulk RNA sequencing

Fig. 1

Graphical overview of prime-seq, highlighting its robustness, sensitivity, affordability, and the validation experiments performed. Cells are first lysed, mRNA is then isolated using magnetic beads, and in turn reverse transcribed into cDNA. Following cDNA synthesis, all samples are pooled, libraries are made, and the samples are sequenced. The protocol has been validated on 17 organisms, including human, mouse, zebrafish, and arabidopsis. Additionally, prime-seq is sensitive and works with low inputs, and the affordability of the method allows one to increase sample size to gain more biological insight. To verify prime-seq’s performance, we first compared prime-seq to TruSeq using the publicly available MAQC-III Study data. We then showed robust detection of marker genes in NPC differentiation and high-throughput analysis of AML-PDX patient samples without compromising the archived samples

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