Fig. 7

Biological function analysis of stool microbiomes of Bhopal and Kerala. a Heatmap of Spearman rank correlation between DA species identified by mbDenoise-zinb-cov and top twenty differential pathways found by LEfSe. k583: PWY-1269:CMP-3-deoxy-D-manno-octulosonate biosynthesis I; k709: PRPP-PWY:superpathway of histidine, purine, and pyrimidine biosynthesis; k660: GLUCONEO-PWY:gluconeogenesis I; k792: PWY-5918:superpathay of heme biosynthesis from glutamate; k60: PWY-6700:queuosine biosynthesis|g_Prevotella.s_Prevotella_copri; k71: PWY-7221:guanosine ribonucleotides de novo biosynthesis|g_Prevotella.s_Prevotella_copri; k128: PWY-2942:L-lysine biosynthesis III|g_Prevotella.s_Prevotella_copri; k97: PWY-6151:S-adenosyl-L-methionine cycle I|g_Prevotella.s_Prevotella_copri; k4: UNINTEGRATED|g_Prevotella.s_Prevotella_copri; k35: PWY-7219:adenosine ribonucleotides de novo biosynthesis|g_Prevotella.s_Prevotella_copri; k110: PWY-5097:L-lysine biosynthesis VI|g_Prevotella.s_Prevotella_copri; k95: PWY-6151:S-adenosyl-L-methionine cycle I; k152: NONMEVIPP-PWY:methylerythritol phosphate pathway I; k109: PWY-5097:L-lysine biosynthesis VI; k127: PWY-2942:L-lysine biosynthesis III; k58: PWY-6700:queuosine biosynthesis; k69: PWY-7221:guanosine ribonucleotides de novo biosynthesis; k911: PWY-5505:L-glutamate and L-glutamine biosynthesis|unclassified; k359: PWY-7229:superpathway of adenosine nucleotides de novo biosynthesis I|unclassified; k556: PWY-7220:adenosine deoxyribonucleotides de novo biosynthesis II|unclassified. b Heatmap of Spearman rank correlation between the three DA species most correlated with function pathways and the remaining DA species detected by mbDenoise-zinb-cov. We used FDR-adjusted P values. *Adjusted P values were between 0.01 and 0.05, **Adjusted P values were less than 0.01. c Flow chart of biological function analysis based on literature research on three DA species and two differential pathways