Fig. 5

Spatial organization of cell type markers is consistent with maintenance of cell type determination in cardiomyocyte differentiation. a Schematic representations of how cell types might grow out during differentiation with and without maintenance of cell expression state (denoted by color). b Representative images of TNNT2, LUM, EPCAM, ISL1, and WT1 single-molecule RNA FISH of cells fixed on day 12 of cardiac differentiation, demonstrating patches of cells (denoted using dotted lines—TNNT2 in green, LUM in red, ISL1 in pink, EPCAM in blue, and WT1 in yellow) with high marker expression. Nuclei in patches counted manually, guided by DAPI expression. Scale bars are 50 μm. c Frequency of patch sizes associated with each marker gene (bars colored to match dotted lines in b). The mean patch size is denoted with a slightly darker colored vertical line. Total number of images and clusters analyzed per marker also indicated on each histogram