Fig. 1

Identification of ALOG paralogs required for flowering transition. A Phylogenetic relationship and expression dynamics of ALOG family members in tomato. EVM, early vegetative meristem; MVM, middle vegetative meristem; LVM, late vegetative meristem; TM, transitional meristem; FM, floral meristem. B Schematic (upper) indicating sgRNAs (red lines) and allelic information (bottom) for TFAM3, TFAM11, respectively. C Representative shoot and typical primary inflorescences from WT and tfam single, double and triple mutants. White arrowheads indicate inflorescences, red arrows indicate vegetative reversions, and white arrows indicate branching events on inflorescences. L, leaf. Scale bars, 2 cm for plants; 0.5 cm for inflorescences. D, E Statistics of flowering time (D) and flower number per inflorescence (E) for WT and tfam single, double, and triple mutants. The flower numbers were quantified from branched and unbranched inflorescences separately. Data are means ± SD (n = 17, 12, 12, 14, 14, 16, 14, 14, 14, for B; n = 12, 14, 8, 15, 12, 8, 13, 14, 13 for C, *P < 0.05, **P < 0.01, ***P < 0.001, Student t-test)