Fig. 4

Single-cell CUT&Tag2for1. a UMAPs representing the low dimensional embedding of cells using inferred Pol2S5p peaks (left), colored by the logarithm of the library size of single cells. b Same as a, using inferred H3K27me3 peaks. c Same as a, using both inferred Pol2S5p and H3K27me3 peaks. d Plot comparing the number of fragments mapping to Pol2S5p and H3K27me3 peaks for H1 and K562 cells (left). UMAP of cells colored by fraction of fragments mapping to H3K27me3 peaks (right). e Heatmap of normalized fragment counts for the top 400 most variable Pol2S5p and H3K27me3 peaks (as rows) from Principal Component 1 across 515 K562 cells and 427 H1 cells (as columns). Results are shown for replicate 1