Fig. 6

PTPRH knockdown in neuroblastoma cell lines increases cellular proliferation and migration. A Change in relative confluence (i.e., confluence at each time point normalized to confluence at time 0) for parental and PTPRH knockdowns (KDs) in SK-N-BE(2) and SK-N-SH cells. Six replicates for each condition were used for the SK-N-BE(2) cell line and SK-N-SH cell line. B Left panels show representative images for scratch wound assays used to measure cellular migration for parental and PTPRH-KD in SK-N-BE(2) and SK-N-SH cells. Right panels show wound closure percentage for parental and PTPRH-KD in SK-N-BE(2) and SK-N-SH cells over 48 h. Each time point had 9 replicates. Error bars indicate +/− one standard deviation around the mean across replicates at each time point. The lines are fits from cubic spline regression using 3 knots, and the p-value is from an F-test for a difference in splines between parental and KD cells