Fig. 7From: Comparative analysis of antibody- and lipid-based multiplexing methods for single-cell RNA-seqPreferable hashing techniques according to our study. TotalSeq antibody hashing is a preferred method for multiplexing different human cell lines, PBMCs, mice spleen, lung, and skin cells. Lipid-modified oligos are better on mice brain cells. The CMO hashing method yields the best results when nuclei are the input sample. CMO cholesterol-modified oligosBack to article page