Fig. 3
From: Comparative analysis of antibody- and lipid-based multiplexing methods for single-cell RNA-seq
Lipid hashing (CMO) outperforms antibody hashing on nuclei. Each column represents a separate hashing method or a replicate. A Hashtag-derived oligo (HTO) matrices were generated using CellRanger, followed by log-transformation and visualised on heatmaps. B Nuclei annotation (4 cell lines) was performed using freemuxlet (gene expression) and visualized on the gene expression UMAP plots. C MULTISeqDemux-annotated nuclei (HTO signal) were matched with the freemuxlet-annotated nuclei. Classification accuracy of every hashing method reported for each cell line