Fig. 5

Discovery of novel peptides and full-length protein isoforms. a Novel peptide MIF confirms translation of an ATG start for RBMS1. b Novel peptide GYA confirms translation of a novel retained intron for FXR1. c Novel peptides ESD and EVR confirms the translation of a novel terminal exon for RABGAP1L. In this case, since the novel peptide maps exclusively to PB.1248.6, the corresponding full-length protein isoform is likely translated. Note that only ESD passed strict manual annotation, but EVR, which passed a 1% FDR in the global MS search, supports the expression of the same terminal exon