Fig. 6

CircRNA alternative circularization generated clustered circRNAs with potential additive functions. a Distribution of circRNA numbers in circRNA clusters defined by alternative circularization events. b Nine circRNA isoforms in circRNA cluster FIP1L1 identified by A-tailing data display distinct expression patterns during HOG differentiation. c Scatter plot shows circRNA cluster complexity and expression changes during HOG differentiation. All dots represent significantly changed circRNA clusters during HOG differentiation. Insignificant circRNA changes are in red. Dot size represents the change of circRNAs number upon HOG differentiation within each cluster. d Cumulative bar plot showed the expression change of each circRNA in circRNA cluster ARHGEF28. IGV view showed a common region where circRNA cluster ARHGEF28 accumulated and significantly enriched during HOG differentiation. DESeq2 was used for circRNA cluster expression comparisons. *P < 0.05. e Schematic diagram showed that a common sequence of circRNA cluster ARHGEF28 (blue) could potentially regulate OL differentiation by sponging miR-454-3p. f Expression of ERBB4 upon HOG differentiation. g Expression of ERBB3 upon HOG differentiation. Cuffdiff was used for circRNA expression comparison. **P < 0.01