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Fig. 5 | Genome Biology

Fig. 5

From: Accurate identification of circRNA landscape and complexity reveals their pivotal roles in human oligodendroglia differentiation

Fig. 5

CircSPATA13 regulated oligodendroglioma differentiation via sponging miR-760. a Scatter plot showed miRNA expression level (x-axis) and significant expression change of their target genes (y-axis) during HOG differentiation. An equal number of randomly selected non-miRNA target genes were used as a negative control. b Cumulative plot showed miR-760 targets were downregulated in HOG differentiation compared with randomly selected non-miRNA targets. The green line stands for log2 fold change of random non-miRNA target genes. The orange line represents log2 fold change of target genes according to the TargetScanHuman database. The blue line represents log2 fold change of most confident target (top 90% according to context++ score) from TargetScanHuman database. Student’s t-test (two-tailed and unpaired) compared top targets versus random targets, and p-values indicated. c Cumulative plot showed pre-mRNA of miR-760 target genes were not affected in HOG differentiation. d Volcano plot showed miRNA expression change upon HOG differentiation. Blue and red dots represent significantly downregulated and upregulated miRNAs during HOG differentiation (DESeq2, FDR < 0.05). Expression of miR-760 was not significantly changed upon HOG differentiation. e Bar plot showed circSPATA13 was downregulated while circPEX6 was upregulated in differentiated HOG cells. Cuffdiff was used for gene expression comparison. **P < 0.01. f A standard curve was constructed to calculate the copy number of circSPATA13 where the x-axis stands for log2 copy number of circSPATA13 and the y-axis stands for Ct value from qPCR. g Expression change of circSPATA13, MYC, HIST1H2BM, linear SPATA13, and HERC6 upon si-circSPATA13 in HOG cell. The t-test (two-tailed and unpaired) was used for gene expression comparison. n = 7, ***P < 0.001, *P <0.05, “NS” indicating no significant change. h CircRNA-miRNA-mRNA network regulating HOG differentiation. Blue and orange represent down- and upregulated circRNA/miRNA/mRNA upon HOG differentiation. Dash lines represent inactivation of upstream regulator promote downstream target or biological process

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