Fig. 5

ABE-induced clustered RNA and DNA A>G SNVs. a An IGV genome browser view showing representative loci with clustered A>G SNVs in transcriptomes. b Ratios of A>G mutations were calculated in flanking 5′ and 3′ 30-bp regions centered at A>G RNA SNV loci. Lines R49bAG_s2 and R49bAG_s3 with RNA mutations and line RCas_s1 with SpCas9 only are shown. c Boxplot showing number of A>G SNVs in the flanking 5′ and 3′ 30-bp regions separately for RNA SNVs in many (3–8) or few (1–2) plants. d IGV genome browser views showing representative SNV loci with flanking A>G SNVs in whole-genome sequencing. e Ratios of clustered SNVs located in genic regions. f Plants with ABEs were classified into two groups: group 1 with clustered SNVs and group 2 without clustered SNVs. Number of SNVs and A>G SNVs, and percentage of A>G SNVs are shown separately for plants in group 1 and plants in group 2. (**) denotes p-value < 0.01, and (***) denotes p-value < 0.001 (one-tailed Wilcoxon test). In IGV genome browser views, the grey bar represents a sequenced nucleotide that is the same as the reference genome, while bars in other colors represent sequenced nucleotides that are partially or totally different from the reference genome: red represents nucleotide A, green represents nucleotide T, orange represents nucleotide G, and blue represents nucleotide C. The height of each color bar represents the relative composition of each nucleotide