Fig. 4

Global dynamic TAD-like structure organization during fiber development. a Venn diagram showing the number of conserved or specific TAD-like structure at four stages: 0 DPA (red), 5 DPA (yellow), 10 DPA (cyan), 20 DPA (blue). b Box plot showing the distribution of structure size in the At and Dt subgenomes. Bar plot showing the structure number. c The average insulation scores at conserved and unique boundaries. The scores in randomly distributed regions were shown as a control. d The expression level of genes located in boundary or interior. e Heatmaps showing representative dynamic TAD-like structure organization in fiber development, with insulation scores shown below. f Bar plot showing the number of expressed genes in dynamic boundaries. For both b and e, two-sided Wilcoxon signed-rank test was used (***P < 2.2 × 10⁻¹⁶)