Fig. 5
IG and TCR transcripts can be identified and paired in 10X R2C2 data. A The workflow to identify antibody (IG) and T cell receptor (TCR) transcripts for each individual cell. B Numbers of cells for which IG or TCR transcripts could be identified and paired. C Schematic of IG identification, composition, and pairing. Each column represents a single B cell. Colored blocks on top of each column indicate whether a cell contains paired IG transcripts (black) and whether an IGH (heavy: gray), IGK (kappa: teal), or IGL (lambda: orange) transcripts were detected. Below the diversity of the detected sequences is shown. Black lines indicate which gene segments were used when an IG sequence was recombined from the germline genome. In CH, it is also shown which isotype(s) we detected (IGHM: black, IGHD: green, IGHA1 or 2: red, IGHG1-4: blue) for each cell