Fig. 1

Data generation and characteristics. A Thousands of peripheral blood mononuclear cells (PBMCs) were processed using the 10X Genomics Chromium Single Cell 3′ Gene Expression Solution. The resulting full-length cDNA was either fragmented for Illumina sequencing or processed using the R2C2 workflow. B After read processing and demultiplexing, the unique molecular identifiers (UMIs) associated with each cellular index (cell) in R2C2 (top) and Illumina (center) datasets are shown as histograms. Cells are ranked by the number of UMIs and colored based on their rank in the R2C2 dataset. Red lines indicate cellular identifiers found in Illumina but not R2C2 data. At the bottom, the UMIs shared between cellular identifiers in Illumina and R2C2 datasets or unique to each dataset are shown as stacked histograms. Cells are ranked by the number of shared UMIs. Data for replicate 1 are shown