Fig. 1
FIONA1 installs m6A modification on U6 snRNA and poly(A)+ RNA. a LC-MS/MS quantification of the m6A/A ratio in nuclear total RNA in 12-day-old Col-0 and fiona1-1 seedlings. b LC-MS/MS quantification of the m6A/A ratios of U6 snRNA in the indicated genotypic plant tissues. c-d Real-time fluorescence amplification curves and bar plot of the threshold cycle (CT) of qPCR showing SELECT results for detecting m6A30 site in U6 snRNA with and without FTO demethylation treatment (c) and in Col-0 and fiona1-1 seedlings (d). Rn is the raw fluorescence for the associated well normalized to the fluorescence of the passive reference dye (ROX). e LC-MS/MS quantification of the m6A/A ratios of poly(A)+ RNA in the indicated genotypic plant tissues. Data are means ± SD for 3 biological replicates × 2 technical replicates. * p < 0.05, ** p < 0.01, *** p < 0.001 by t test (two-tailed). ns, no significance