Fig. 6

Functional analysis of two cheRNAs. A Expression patterns of OsCHELIN2084 (left) and OsCHENAT1709 (right). Values are the means ± SD (n = 3 replicates, normalized against ACTIN2). B Callus at 20 days after induction (DAI) and at 25 days after differentiation (DAD) from DJ-WT, chelin2084-T, ZH11-WT, and chenat1709-T from left to right. Scale bars, 1 mm. C Callus size analysis during callus induction. Values are the means ± SD (n = 45 calli). D Panicles (top) and grains (middle) of DJ-WT and chelin2084-T, and whole ZH11 and chenat1709-T plants (bottom). Scale bars, 3 cm for panicles, 1 cm for grains and 15 cm for plants. E Statistical analysis of tiller number. Values are the means ± SD (n = 11 plants). F Schematic diagram of the relative locations of OsCHELIN2084 and its neighboring genes LOC_Os08g35070 and LOC_Os08g35090. G Relative expression levels of LOC_Os08g35070, and LOC_Os08g35090 in WT and chelin2084-T callus and differentiated callus. Values are the means ± SD (n = 3 replicates, normalized against ACTIN2). In E and G, significant differences were identified at 5% (*) and 1% (**) probability levels using two-tailed paired t-test