Fig. 3From: Statistics or biology: the zero-inflation controversy about scRNA-seq dataA toy example showing how the PCR amplification may result in sampling zeros. Five genes have their cDNAs amplified by PCR. After the non-linear amplification, their relative proportions change. If the sequencing depth is limited to 52 reads, the first gene has sampling zeros in three out of five hypothetical sequencing experimentsBack to article page