Fig.5
A proposed molecular diagram of the metabolic pathway comprising of the genes associated with ORTs in C. oelifera. The biochemical process of enzymes and functions are on the basis of previous reports [23, 24]. A The de novo biosynthesis process of free fatty acids in plastid; B fatty acids modification, acyl editing and TAG assembly in endoplasmic reticulum; C and D oil storage, transportation and breakdown in cytosol and plasma membrane. The key enzymes of the oil metabolism pathway are indicated in the light red boxes. The squares and hexagons are indicating the identification of genes in GWAS or qGWAS, respectively. Colored squares and circles are presenting the significant associations of different oil traits. The yellow circle indicates the oil body. C16:0, palmitic acid; C16:1, palmitoleic acid; C18:0, stearic acid; C18:1, oleic acid; C18:2, linoleic acid; C18:3, linolenic acid; C20:1, cis-11-eicosenoic acid; Acyl-CoA, acyl-coenzyme A; ACC, acetyl-CoA carboxylase; FabD, malonyl CoA:ACP transacylase; KAS I, II, III, β-ketoacyl-[acyl carrier protein] synthase I, II, III; SAD, stearoyl ACP desaturase; FatA and FatB, fatty acyl-ACP thioesterase A, B; FFA, free fatty acid; LACS, long-chain acyl-CoA synthase; LPCAT, acyl-CoA: lysophosphatidylcholine acyltransferase; PC, phosphatidylcholine; FAD2 and FAD6, fatty acid Delta-12 desaturase; FAD3 and FAD7, fatty acid Delta-15 desaturase; PUFA, polyunsaturated fatty acid; G3P, glycerol-3 phosphate; GPAT, G3P acyltransferase; LPA, lyso-phosphatidic acid; LPAAT, LPA acyltransferase; PA, phosphatidic acid; PAP, phosphatidic acid phosphatase; DAG, diacylglycerol; DGAT, diacylglycerol acyltransferase; PLC and PLD, phospholipase C and D; DGK, diacylglycerol kinase; SDP1, sugar-dependent triacylglycerol lipase; DGL, diacylglycerol lipase; lip1, monoacylglycerol lipase; GLPK, Glycerol kinase; LTPG1, non-specific lipid transfer protein GPI-anchored 1; GDSL and GDL57, GDSL esterase/lipase