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Fig. 5 | Genome Biology

Fig. 5

From: CRISPR guides induce gene silencing in plants in the absence of Cas

Fig. 5

The Cas13 DR sequence is not required to elicit GIGS. a Schematic representation of modified PDS m-guide1 sequences where the LbuCas13a DR was replaced with Arabidopsis miR170 stem-loop (stem-loop, 24 nt), the miR170 without the four base pair stem (loop, 16 nt), a random sequence (random, 20 nt), and without any intervening sequence (contiguous). b Representative images of N. benthamiana leaves after 2 weeks of TRV systemic delivery. The guide delivered for each TRV construct is labeled above or below the image. The bottom row shows leaves that all expressed a version of PDS m-guide 1. c Chlorophyll content (leaf greenness) measured with SPAD meter 2 weeks after systemic delivery of crRNA. d Relative PDS transcript levels quantified using qPCR, compared between the infiltration control (TRVempty) and each treatment. Statistical comparisons were made using one-way ANOVA with multiple comparisons of treatments by means of Tukey performed using honestly significant difference. Treatments with the same letter are not significantly different

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