Fig. 4

Structural variant calling performance. a Genome-browser view shows read alignments at deletion for ONT PromethION (green track), HiSeq X Ten/PCR-free (purple track), and MGISEQ-2000 (yellow track) at heterozygous deletion (in white). The histogram shows the depth of coverage at each position, with aligned reads shown below. b Relative frequency of correctly called SVs, with breakpoints successfully identified, relative to sequencing coverage and bioinformatic tool. a, b The colors represent the different sequencing technologies/preparation methods used to generate each of the seven sequenced libraries, such as HiSeq X Ten/PCR-free (purple), HiSeq 2500/PCR-based (red), NextSeq 500/PCR-based (blue), MGISEQ-2000 (yellow), and ONT PromethION (green)