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Fig. 4 | Genome Biology

Fig. 4

From: Species-resolved sequencing of low-biomass or degraded microbiomes using 2bRAD-M

Fig. 4

Comparison of taxonomic profiles and desired sequencing depth of 2bRAD-M, 16S, and WMS in fecal samples. a–c Comparison of taxonomic profiles at the genus level between 16S rRNA and 2bRAD-M. d–f Comparison of taxonomic profiles at the species level between 2bRAD-M and WMS. To perform the comparison in a fair manner, we extracted 2bRAD reads from WMS data and then used these WMS-originated 2bRAD reads as input for the 2bRAD-M computational pipeline. Then, species abundance generated by 2bRAD-M (using 2bRAD-M sequencing data) is used as the X-axis while the species abundance generated by 2bRAD-M (using WMS data) as the Y-axis for the scatter plot. g The rarefaction analysis of 2bRAD-M and WMS samples. The species-level compositions in the subsampled data at each given sequencing depth were compared to the pre-rarefaction result for each method

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