Fig. 2
From: Species-resolved sequencing of low-biomass or degraded microbiomes using 2bRAD-M
Benchmark measurements of 2bRAD-derived taxonomic profile. a Simulated microbial community data consisting of 50 microbes were profiled by each of the 16 type IIB restriction enzymes. The scatter plots indicate the correlation of the taxonomic abundance estimated from 2bRAD-M with the expected abundance for each enzyme. The percentage number indicated in each plot represents the average genome coverage (compare to the original 50 microbial genomes) after digesting by the enzymes. b Performance comparison of 2bRAD-M with Kraken2, Bracken, MetaPhlAn2, and mOTUs2 based on 25 simulated communities. Two types of abundance are used as the ground truth of the simulation data to evaluate the performance: sequence abundance is used to evaluate Bracken and Kraken2, while taxonomic abundance is used to evaluate 2bRAD-M, mOTUs2, and MetaPlAn2