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Fig. 6 | Genome Biology

Fig. 6

From: Quantitative-enhancer-FACS-seq (QeFS) reveals epistatic interactions among motifs within transcriptional enhancers in developing Drosophila tissue

Fig. 6

Epistatic interactions identified by QeFS. A Synergistic epistasis is observed when mutating two classes of sites produces a greater effect than a multiplicative model predicts from the results of single class mutants, as when Deaf1 and CG12236-PB sites are mutated in ChIPCRM5432. B, C Alleviating epistasis describes an effect smaller than that predicted by a multiplicative model, and can be subclassified [51]. In this study, we observed B complementary epistasis, where the severity of effects of the double mutant equals those of each of the single mutants, as when Deaf1 and CG12236-PB sites are mutated in ChIPCRM2847, and C complete suppression, where the effects of mutating one motif suppress the effects of mutating another motif (i.e., severity of effects of the double motif mutant A+B = severity of effects of the single motif mutant B < severity of effects of the single motif mutant A), such as Schlank site mutations do to Deaf1 site mutations in CBP2862. D Sign epistasis describes a situation wherein the direction of the effect of mutating a class of sites is reversed in the context of mutations in another class of sites, as we observed when mutating ZIPIC and Deaf1 sites in CG7759_33

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