Fig. 5

Effects of combinatorial motif mutations on enhancer activity. A Mutating Deaf1 and CG12236-PB sites together in the ChIPCRM5432 enhancer reduces activity more than predicted under a multiplicative model. B A similar epistatic interaction between these two classes of binding site is observed by whole embryo RT-qPCR in the ChIPCRM2000 enhancer. C Mutating Deaf1 and CG12236-PB sites together in the ChIPCRM2847 enhancer causes no additional reduction in activity as compared to mutating either class of site alone. D Schlank site mutation reduces the effect of mutating Deaf1 and CG12236-PB sites in this enhancer. E Mutating Schlank sites in the CBP2862 enhancer increases expression in the context of Deaf1 site mutations but not in the wild type enhancer. F Mutating Schlank sites decreases expression from the CBP2862 enhancer when the single ZIPIC site is also mutated. G Mutating Deaf1 and ZIPIC sites in the CG7759_33 enhancer reduces its activity, although neither class of site mutation reduces its activity alone. Asterisks and error bars are as in Figs. 2 and 3; binding site abbreviations are as in Fig. 1