Skip to main content
Fig. 5 | Genome Biology

Fig. 5

From: Reprogramming of RNA silencing triggered by cucumber mosaic virus infection in Arabidopsis

Fig. 5

Validation of vsiRNA biological activity. A PARE read profile relative to the predicted cleavage position identified by PAREsnip for AT4G21210 (RP1). B Alignment of the vsiRNA to the predicted target position for AT4G21210 (RP1). Cleavage positions identified by 5′ RACE are indicated with arrows, with the number of clones identified at each specific position relative to the total number of clones analyzed indicated. A PCR-amplified 5′ RACE product for AT4G21210 (RP1) specific of CMV-infected tissues is shown in the image. C Diagram of the design for the constructs used for transient expression analysis. D Transient expression of the target sequence of AT4G21210 in N. benthamiana showed by representative pictures of the intensity of the GFP in mock and infected leaves and their expression levels measured by RT-qPCR. Error bars depict standard deviation normalized to the average mock values, and p values are indicated in the comparisons and indicate the result of an unpaired t test with 2 tails

Back to article page