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Fig. 1 | Genome Biology

Fig. 1

From: Reprogramming of RNA silencing triggered by cucumber mosaic virus infection in Arabidopsis

Fig. 1

CMV-derived sRNAs affect endogenous sRNA proportions. A Representative pictures of mock and CMV-infected Arabidopsis Col-0 at 20 dpi. B Northern blot detection of vsiRNAs, miR168, and U6. U6 was used as a loading control. 20 μg of total RNA was loaded on each lane. C Proportion of CMV sRNAs vs endogenous Arabidopsis sRNAs in CMV-infected sRNA libraries. D Size distribution of the whole mapped sRNAs for mock and CMV-infected libraries. E Categorization in different classes of sRNA for mock and CMV-infected samples. F Proportion of 21-, 22-, and 24-nt sRNAs mapping to miRNAs, TEs, and mRNAs in mock and CMV-infected samples. G Scatterplot of sRNA accumulation (log10 of RPMs) for individual miRNA families, TEs, and mRNAs. Elements that accumulate 2-fold or higher sRNAs in CMV samples are highlighted in blue while elements that accumulate 2-fold or higher in mock samples are highlighted in red. Two bioreplicates from sRNA libraries were generated and analyzed

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