Fig. 6
From: Knockout of circRNAs by base editing back-splice sites of circularized exons
KO of circRNA with base changes by BE that requires NG PAM or NRN/NYN PAM. A Only one or none sgRNA could be designed for base changes of 5′ (b)ss of circSPECC1 and circARCN1 by CBE that requires NGG PAM. B Two or one sgRNA could be designed for base changes of 5′ (b)ss of circSPECC1 and circARCN1 by CBE that requires NG PAM. C Seven or six sgRNAs could be designed for base changes of 5′ (b)ss of circSPECC1 and circARCN1 by nSpRY-conjugated BE that requires NRN/NYN PAM. D In silico screening of circRNAs with predominantly back-spliced novel exons, targeted by hA3A-eBE-Y130F with NG PAM at back-splice sites. E In silico screening of circRNAs with predominantly back-spliced novel exons, targeted by nSpRY-conjugated BE that requires NRN/NYN PAM at back-splice sites