Fig. 4

Co-regulation and replicating empirical 10x-Neuro data. a Pearson’s correlation between mean aggregated expression of randomly paired (gray, n = 1000) and co-regulated (blue, n = 54) eGenes across 100 individuals. The average correlation for each type designated by the large point. b, c Comparison of 10x-Neuro and splatPop simulated data using the replication approach. Eight individuals in the same batch and 358 genes meeting a minimum expression threshold (> 10 counts across all cells) were included. b Pairwise Pearson’s correlation between individuals across genes, with expression for each gene for each individual calculated as the mean aggregated log counts across cells. Individuals are ordered by hierarchical clustering of the empirical data. c Relationship between mean aggregated log counts across cells for each individual for each gene from empirical (x-axis) and simulated (y-axis) data, colored by individual. The diagonal is shown with the black line, the linear regression line is dashed blue, and the Pearson’s correlation between empirical and simulated aggregated logcounts across all genes and all individuals is shown in blue