Fig. 2

The termination landscape in Arabidopsis. a Schematic diagram shows the definition of termination window presented in this study. Reads with 3′ end located more than 50 nt downstream of poly(A) site are used to define termination window. The readthrough distance is calculated as the distance between the poly(A) site and the 3′ end of reads. The median readthrough distance at the corresponding gene is used to represent its termination window size. b Comparison of termination window size quantified by either the median or longest readthrough distance. The gene model is shown at the top and is zoom-in to highlight the region from 200 nt upstream of the poly(A) site to the longest readthrough distance. The boxplots show the readthrough distance distribution for all reads in the region. pA, poly(A) site (also in Fig. 2f, g, and Fig. 4a, d). c, d Scatter plots for the longest (c) and median (d) readthrough distance per gene between replicates (a minimum of 15 readthrough reads for calculating the termination window size). The Pearson’s correlation coefficient and the number of genes (n) are shown. e The distribution of termination window size among genes. f, g Examples of genes with short and long termination windows, respectively