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Fig. 1 | Genome Biology

Fig. 1

From: A cis-regulatory-directed pipeline for the identification of genes involved in cardiac development and disease

Fig. 1

Pipeline for predicting genes essential for cardiac development and disease. (1) Organ-specific promoters and enhancers gleaned from H3K4me3 and H3K4me1/H3K27ac ChIP-seq analysis respectively. (2) Heart-specific promoters and enhancers selected from the heart-specific subset (panel 1: green ticks) were (3) processed through GREAT [18]. (4) RNA-seq data were used for filtering genes that are expressed in the heart. (5) The gene regulatory network was constructed from the STRING database and arranged using a force-directed layout [19]. (6) Functional validations were performed by heart-specific targeted knock-down in Drosophila melanogaster. Related to Additional file 1: Fig S1

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Genome Biology

ISSN: 1474-760X

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