Fig. 2
From: An accessible, efficient and global approach for the large-scale sequencing of bacterial genomes
LITE (Low Input, Transposase Enabled) pipeline for library construction. The DNA was extracted using a protocol based on the MagAttract HMW DNA isolation kit (Qiagen). Library construction was performed by tagmentation using Nextera tagmentation kit, size selected on a BluePippin, and quantified using a High Sensitivity BioAnalyzer kit (Agilent) and Qubit dsDNA HS Assay (ThermoFisher). Genome sequencing of “super pools” was performed in a HiSeqTM 4000 (Illumina) system, and re-sequencing in NovaSeqTM 6000 (Illumina) when needed, both with a 2 × 150 bp paired ends read metric