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Fig. 4 | Genome Biology

Fig. 4

From: A systems genetics approach reveals PbrNSC as a regulator of lignin and cellulose biosynthesis in stone cells of pear fruit

Fig. 4

PbrNSC involved in stone cell formation. a Expression of PbrNSC in different tissues of “Dangshansuli” plants determined by qPCR. Each value is mean ± SD (n = 3 biological replicates). b Expression of PbrNSC in stone cells and flesh tissues of pear fruit at 35 DAFB. Each value is mean ± SD (n = 3 biological replicates). c Transient assays using PbrNSC overexpression and silencing constructs in “Dangshansuli” fruit at 35 DAFB. Fruit flesh was stained with Wiesner reagent, and images were taken 10 days after agro-infiltration. d, e Lignin (d) and cellulose (e) contents in the fleshy tissue around the infiltration sites. Each value is mean ± SD (n ≥ 12 biological replicates). f, g Lignin (f) and cellulose (g) contents of inflorescence stem of 8-week-old WT and transgenic plants. Each value is mean ± SD (n ≥ 3 biological replicates). h S- and G-lignin contents of inflorescence stems of 8-week-old WT and transgenic plants. Each value is mean ± SD (n = 3 biological replicates). i Cross-sections of inflorescence stems of 8-week-old plants stained with different reagents to detect lignin and cellulose deposition. The Mäule reagent specifically binds to S-lignin monomers; the Wiesner reagent binds to G-lignin monomers. Lignin autofluorescence of cross-sections was detected under UV light. Cross-sections were stained with the fluorescent dye Congo Red (for cellulose). Bar = 200 μm. xy, xylem; if, interfascicular fiber cell. A representative picture from each line is shown. j Transmission electron micrographs of cross-sections of interfascicular fiber and vessel cells in 8-week-old inflorescence stems. Bar = 5 μm. k, l Quantitative analysis of SCW thickness in interfascicular fiber (k) and vessel (l) cells. Three plants in each genotype and more than 20 cells in each plant were analyzed. k n = 499; l n = 474. Student’s t-test; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001

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